Title
Podocyturia as diagnostic and prognostic marker of preeclampsia
Creator
Garović, Vesna
Copyright date
2016
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
English
Cobiss-ID
Committee report
Theses Type
Doktorska disertacija
description
Datum odbrane: 19.06.2017.
Other responsibilities
mentor
Plećaš, Darko, 1953-
član komisije
Egić, Amira, 1957-
član komisije
Simić, Tatjana, 1964-
član komisije
Radović, Milan, 1965-
član komisije
Milić, Nataša, 1974-
član komisije
Babić, Marko
Academic Expertise
Medicinske nauke
University
Univerzitet u Beogradu
Faculty
Medicinski fakultet
Alternative title
Dijagnostički i prognostički značaj podociturije kod žena sa preeklampsijom
Publisher
[V. D. Garović]
Format
58 listova
description
Medicine - Gynecology and obstetrics / Medicina - Ginekologija i akušerstvo
Abstract (en)
Preeclampsia is a pregnancy-specific disorder clinically characterized by hypertension
and proteinuria that occurs after 20 weeks of gestation. Affecting 5% of pregnancies, it remains
one of the leading causes of both maternal and fetal morbidity and mortality worldwide.
Preeclampsia covers a spectrum of conditions, with eclampsia (its convulsive form) and HELLP
syndrome representing its most severe forms. Despite recent advances in the field of
angiogenesis and anti-angiogenesis in preeclampsia, urine and serum measurements of
circulating angiogenic proteins have not provided a reliable screening tool for preeclampsia with
current techniques. Emerging evidence suggests that podocyte plays a critical role in the
evolution of kidney injury in this disorder. Studies of human tissue show that the expressions of
podocyte-specific proteins (including nephrin and synaptopodin) are severely affected by
preeclampsia. In addition, the detection of podocyte products and live podocytes in the urine
(podocyturia) may serve as clinically useful tools for prediction and diagnosis of preeclampsia.
Identification of podocytes using the techique of culturing and staining of urinary sediments is
time consuming and requires special expertise. To overcome these limitations, a new technique
using Mass spectrometry was developed that allows to confirm the presence of urinary podocytes
trough identification of the podocyte-specific proteins. In addition, it was shown that women
with preeclamptic pregnancies demonstrate signs of small vessel disease and less favorable
cardiovascular risk profile both at the time of delivery and years after their affected pregnancies.
One possible mechanism for this relationship is that hypertensive pregnancy disorders
(preeclampsia, in particular) and CVD share several common risk factors (obesity, diabetes
mellitus, and renal disease) or, alternatively, hypertension in pregnancy may induce long-term
metabolic and vascular abnormalities that may increase and overall risk for cardiovascular
disease later in life...
Abstract (sr)
Preeklampsija je oboljenje koje se javlja u trudnoći i klinički se karakteriše pojavom
hipertenzije i proteinurije nakon dvadesete nedelje trudnoće. Javlja se u 7 – 10% svih trudnoća i
čini vodeći uzrok mortaliteta i morbiditeta trudnica i fetusa. Klinička slika ovog oboljenja varira
od lakih do najtežih formi, koje mogu da se manifestuju pojavom konvulzija tj. razvojem
eklampsije, kao i razvojem hemolize, poremećaja funkcije jetre i koagulacije u okviru tzv. HELP
sindroma. Uprkos nedavnom napretku u razumevanju uloge proteina angiogeneze u
patofiziologiji preeklampsije, postojeći dokazi iz kliničkih studija još uvek su nedovoljni za
korišćenje u ranom otkrivanju trudnoća koje su pod rizikom za razvoj preeklampsije. Prvi
objavljen rad iz serije radova o dijagnostičkom i prognostičkom značaju podociturije kod žena sa
preeklampsijom pokazao je da je pojava proteinurije u preeklampsiji povezana sa podociturijom,
odnosno pojavom i gubitkom glomerularnih epitelnih ćelija (podocita) u mokraći, kao i sa
promenama u ekspresiji strukturnih proteina (nefrina i sinaptopodina) ovih ćelija. Ovi klinički
podaci uputili su na zaključak da se podociturija javlja pre proteinurije i hipertenzije, te da može
da posluži kao rani pokazatelj i dijagnostički marker ovog oboljenja. S obzirom da je tehnika
identifikacije podocita koja se bazira na kulturi sedimenta urina komplikovana i da zahteva
posebnu obučenost za interpretaciju rezultata, u nastavku studije razvijena je nova tehnika
identifikacije podocita koja se bazira na masnoj spektrometriji. Ovom tehnikom, potvrđeno je da
se podociturija moze dijagnostikovati identifikacijom peptida koji je specifičan za podocin...
Authors Key words
preeclampsia, proteinuria, podocyturia, glomerular epithelial cells, cardiovascular
disease, women's health
Authors Key words
preeklampsija, proteinurija, podociturija, glomerularne epitelijalne ćelije,
kardiovaskularna bolest, zdravlje žena
Classification
618.3-008(043.3)
Type
Tekst
Abstract (en)
Preeclampsia is a pregnancy-specific disorder clinically characterized by hypertension
and proteinuria that occurs after 20 weeks of gestation. Affecting 5% of pregnancies, it remains
one of the leading causes of both maternal and fetal morbidity and mortality worldwide.
Preeclampsia covers a spectrum of conditions, with eclampsia (its convulsive form) and HELLP
syndrome representing its most severe forms. Despite recent advances in the field of
angiogenesis and anti-angiogenesis in preeclampsia, urine and serum measurements of
circulating angiogenic proteins have not provided a reliable screening tool for preeclampsia with
current techniques. Emerging evidence suggests that podocyte plays a critical role in the
evolution of kidney injury in this disorder. Studies of human tissue show that the expressions of
podocyte-specific proteins (including nephrin and synaptopodin) are severely affected by
preeclampsia. In addition, the detection of podocyte products and live podocytes in the urine
(podocyturia) may serve as clinically useful tools for prediction and diagnosis of preeclampsia.
Identification of podocytes using the techique of culturing and staining of urinary sediments is
time consuming and requires special expertise. To overcome these limitations, a new technique
using Mass spectrometry was developed that allows to confirm the presence of urinary podocytes
trough identification of the podocyte-specific proteins. In addition, it was shown that women
with preeclamptic pregnancies demonstrate signs of small vessel disease and less favorable
cardiovascular risk profile both at the time of delivery and years after their affected pregnancies.
One possible mechanism for this relationship is that hypertensive pregnancy disorders
(preeclampsia, in particular) and CVD share several common risk factors (obesity, diabetes
mellitus, and renal disease) or, alternatively, hypertension in pregnancy may induce long-term
metabolic and vascular abnormalities that may increase and overall risk for cardiovascular
disease later in life...
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