Title
Genotyping, antibiotic resistance and biofilm formation of Stenotrophomonas maltophilia clinical isolates from Serbia
Creator
Madi, Haowa, 1986-
Copyright date
2017
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
English
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 08.09.2017.
Other responsibilities
mentor
Lozo, Jelena, 1974-
član komisije
Jovčić, Branko
član komisije
Kojić, Milan
član komisije
Lozo, Jelena, 1974-
Academic Expertise
Prirodno-matematičke nauke
Academic Title
-
University
Univerzitet u Beogradu
Faculty
Biološki fakultet
Alternative title
Генотипизација, резистенција на антибиотике и формирање биофилма клиничких изолата Stenotrophomonas maltophilia из Србије
Publisher
[H. Madi]
Format
87 listova
description
Molecular biology - Molecular microbiology / Молекуларна биологија - Молекуларна микробиологија
Abstract (en)
Stenotrophomonas maltophilia is an environmental bacterium and an
opportunistic pathogen usually associated with healthcare-associated
infections, which has recently been recognized as a global multidrug resistant
organism. The aim of this study was genotyping and physiological
characterization of S. maltophilia collected during the routine health care at The
Institute for Mother and Child Health Care of Serbia “Dr Vukan Čupić“. It is the
large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national
reference cystic fibrosis (CF) center for pediatric and adult patients.
We characterized 88 S. maltophilia strains, 42 strains of cystic fibrosis (CF)
and 46 strains of non-cystic fibrosis (non-CF) origin isolated from 2013 to 2015
in order to investigate their genetic relatedness and phenotypic traits.
Genotyping was performed using sequencing of 16S rRNA gene, Pulse Field
Gel Electrophoresis (PFGE) and Multi locus sequencing typing (MLST) analysis.
Sensitivity to five relevant antimicrobial agents was determined, namely
trimethoprim/sulfamethoxazole (TMP/SMX), chloramphenicol, ciprofloxacin,
levofloxacin and tetracycline. Surface characteristics, motility, biofilm formation
and adhesion to mucin were tested in all strains. In addition, influence of
different factors (temperature, pH, agitation and CO2) on biofilm formation,
kinetics of selected biofilm producers and effect of TMP/SMX on formed
biofilm were analyzed. Statistical approach was used to determine correlations
between obtained results.
All analyzed clinical isolates belong to S. maltophilia species with identity
ranging from 95% to 99% with S. maltophilia strains from the NCBI database
what was confirmed by sequencing of PCR products of amplified 16S rRNA
gene...
Authors Key words
Stenotrophomonas maltophilia, healthcare-associated infections,
trimethoprim–sulfamethoxazole, biofilm, PFGE, MLST
Authors Key words
Stenotrophomonas maltophilia, нозокомијалнa инфекција,
триметоприм-сулфаметаксазол, biofilm, PFGE, MLST
Classification
[579.253:[579.841:[574.58+616-78]]:[579.61:616-092](497.11)(043.3)
Type
Tekst
Abstract (en)
Stenotrophomonas maltophilia is an environmental bacterium and an
opportunistic pathogen usually associated with healthcare-associated
infections, which has recently been recognized as a global multidrug resistant
organism. The aim of this study was genotyping and physiological
characterization of S. maltophilia collected during the routine health care at The
Institute for Mother and Child Health Care of Serbia “Dr Vukan Čupić“. It is the
large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national
reference cystic fibrosis (CF) center for pediatric and adult patients.
We characterized 88 S. maltophilia strains, 42 strains of cystic fibrosis (CF)
and 46 strains of non-cystic fibrosis (non-CF) origin isolated from 2013 to 2015
in order to investigate their genetic relatedness and phenotypic traits.
Genotyping was performed using sequencing of 16S rRNA gene, Pulse Field
Gel Electrophoresis (PFGE) and Multi locus sequencing typing (MLST) analysis.
Sensitivity to five relevant antimicrobial agents was determined, namely
trimethoprim/sulfamethoxazole (TMP/SMX), chloramphenicol, ciprofloxacin,
levofloxacin and tetracycline. Surface characteristics, motility, biofilm formation
and adhesion to mucin were tested in all strains. In addition, influence of
different factors (temperature, pH, agitation and CO2) on biofilm formation,
kinetics of selected biofilm producers and effect of TMP/SMX on formed
biofilm were analyzed. Statistical approach was used to determine correlations
between obtained results.
All analyzed clinical isolates belong to S. maltophilia species with identity
ranging from 95% to 99% with S. maltophilia strains from the NCBI database
what was confirmed by sequencing of PCR products of amplified 16S rRNA
gene...
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