Title
Characterization of the mechanisms of photoreceptor degeneration during retinal detachment; the evaluation of postoperative anatomical and functional recovery
Creator
Potić, Jelena, 1985- 14419303
Copyright date
2018
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
English
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 28.11.2018.
Other responsibilities
mentor
Arsenijevic, Yvan, biolog. 16243815
mentor
Wolfensberger, Thomas J., 1963- 16250471
član komisije
Bumbaširević, Vladimir, 1951- 12336999
član komisije
Pekmezović, Tatjana, 1964- 12668519
član komisije
Stojković, Milenko, 1966- 12728167
član komisije
Vuković, Dragan, 1959- 12539239
član komisije
Munier, Francis, 1958- 16267367
Academic Expertise
Medicinske nauke
University
Univerzitet u Beogradu
Faculty
Medicinski fakultet
Alternative title
Karakterizacija mehanizama degeneracije fotoreceptora nakon ablacije retine; evaulacija anatomskog i funkcionalnog postoperativnog oporavka
Publisher
[J. R. Potić]
Format
160, [19] listova
description
medicine - epidemiology/ophthalmology/cellular biology / медицина
- епидемиологија/офталмологија/ћелијска биологија
Abstract (en)
Rhegmatogenous retinal detachment (RD) has diagnostically been divided into
macula-OFF (OFF) or macula-ON (ON). Currently the only treatment for RD is surgery.
Despite good anatomical surgical success in majority of cases, visual recovery varies a lot.
Reduction in visual acuity (VA) has a direct effect on patient’s quality of life (QoL). To date,
few studies have reported QoL measures in patients with RD. The degree of visual loss is
proportional to the degree of photoreceptor loss, which has been impossible to assess in-vivo
until recently. If foveal photoreceptor alterations following RD can be observed and
monitored using adaptive optics fundus camera (AO), and if these correlate with visual
impairment, there is a strong case for a clinical application of this device. We previously
identified that cell cycle proteins are reactivated in various rodent models of inherited retinal
dystrophies as well as during light damage; this mechanism might overlap in RD as well.
Therefore, we investigated human retina to have a better comprehension of the cell death
mechanisms that may occur in patients affected by RD. The first step to develop an effective
therapeutic agent is to determine the underlying disease mechanisms to identify the most
appropriate means for intervention.
Aims: The aims of this study were: to describe the demographics and primary outcome of
patients with RD following surgery with respect to the macular status, and to determine risk
factors for macular involvement; to measure and compare preoperative and postoperative QoL
in OFF and ON RD patients; to report new distinct type of RD: hemi-macular detachment
(HMD); to evaluate the changes in choroidal thickness (CT) pre- and postoperatively in RD
and among different RD types; to quantify the changes in cone density and topography using
AO in RD, and to correlate (or not) these changes, macular involvement and changes in best
corrected VA (BCVA) [logMAR]; and to reveal whether apoptosis, cell cycle reentry and
epigenetic modifications, are also involved in an in vitro model of human RD, which was
shown to activate different pathways during the initiation of photoreceptor degeneration.
Methods: This prospective, observational mono-centric cohort study was conducted at the
Jules-Gonin Eye Hospital, from February 2015 until March 2017. 204 patients with primary
RD were enrolled but 194 were analyzed in the study...
Abstract (sr)
Регматогене аблације ретине (АР) се анатомски могу поделити на АР са захваћеном
макулом (макула-ОФ) и оне са незахваћеном макулом (макула-ОН). Тренутно једини
начин лечења АР је хирургија. Међутим, и поред доброг анатомског успеха операције,
у већини случајева, опоравак вида је веома различит. Смањење видне оштрине (ВО)
[logMAR] има директан утицај на квалитет живота (КвЖ) пацијената. До данас, ретке
су студије које су истраживале КвЖ код болесника са АР. Степен губитка вида
пропорционалан је степену губитка фоторецептора, што није било могуће испитати invivo
(уживо) до скоро. Уколико алтерације фоторецептора током АР могу бити
забележене користећи „адаптив оптикс фундус камеру (AO)“, и уколико се ове промене
могу повезати са губитком вида, отварају се врата клиничкој примени АО апарата.
Раније је доказано да се протеини ћелијског циклуса реактивирају у различитим
експерименталним моделима ретиналних дистрофија на глодарима, као и у току
оштећења ретине светлости. Постоји могућност да се ови механизми преклапају и у
моделу АР. Стога, у овој студији је рађено истраживање на хуманој ретини у циљу
бољег разумевања механизама ћелијске смрти који се одигравају код болесника са АР.
Први корак ка развоју ефикасног лека је да се дефинише механизам болести у циљу
препознавања најбољег начина лечења.
Циљ
Циљеви студије су: дефинисати демографске карактеристике болесника са АР, као и
примарни исход хирургије АР у односу на захваћеност макуле, дефинисати факторе
ризика за захваћеност макуле са АР; анализирати преоперативни и постоперативни
КвЖ код пацијената са ОН или ОФ АР; истакнути постојање посебне подгрупе АР:
хеми-макуларна аблација ретине (ХМАР); анализирати промене у дебљини хориоидее
(ДХ) пре и после операције АР и између различитих типова АР; квантификација
промена у густини чепића и топографији фоторецептора код АР помоћу АО, као и
повезати ове промене са захваћености макуле и променама у ВО; и испитати да ли су
апоптоза, поновни улазак фоторецептора у ћелијски цуклус и епигенетске
модификације такође присутни у in vitro експерименталном моделу хумане ретине...
Authors Key words
retinal detachment; macular status; adaptive optics; cone mosaic; choroidal
thickness; EnFace OCT; vision-related quality of life; cell death; in vitro human retina
Authors Key words
аблација ретине; макуларни статус; адаптив оптикс; мозаик чепића;
дебљина хориоидее; АнФас ОЦТ; квалитет живота; ћелијска смрт; in vitro људска
ретина
Classification
617.735-007.281-089-03(043.3)
Type
Tekst
Abstract (en)
Rhegmatogenous retinal detachment (RD) has diagnostically been divided into
macula-OFF (OFF) or macula-ON (ON). Currently the only treatment for RD is surgery.
Despite good anatomical surgical success in majority of cases, visual recovery varies a lot.
Reduction in visual acuity (VA) has a direct effect on patient’s quality of life (QoL). To date,
few studies have reported QoL measures in patients with RD. The degree of visual loss is
proportional to the degree of photoreceptor loss, which has been impossible to assess in-vivo
until recently. If foveal photoreceptor alterations following RD can be observed and
monitored using adaptive optics fundus camera (AO), and if these correlate with visual
impairment, there is a strong case for a clinical application of this device. We previously
identified that cell cycle proteins are reactivated in various rodent models of inherited retinal
dystrophies as well as during light damage; this mechanism might overlap in RD as well.
Therefore, we investigated human retina to have a better comprehension of the cell death
mechanisms that may occur in patients affected by RD. The first step to develop an effective
therapeutic agent is to determine the underlying disease mechanisms to identify the most
appropriate means for intervention.
Aims: The aims of this study were: to describe the demographics and primary outcome of
patients with RD following surgery with respect to the macular status, and to determine risk
factors for macular involvement; to measure and compare preoperative and postoperative QoL
in OFF and ON RD patients; to report new distinct type of RD: hemi-macular detachment
(HMD); to evaluate the changes in choroidal thickness (CT) pre- and postoperatively in RD
and among different RD types; to quantify the changes in cone density and topography using
AO in RD, and to correlate (or not) these changes, macular involvement and changes in best
corrected VA (BCVA) [logMAR]; and to reveal whether apoptosis, cell cycle reentry and
epigenetic modifications, are also involved in an in vitro model of human RD, which was
shown to activate different pathways during the initiation of photoreceptor degeneration.
Methods: This prospective, observational mono-centric cohort study was conducted at the
Jules-Gonin Eye Hospital, from February 2015 until March 2017. 204 patients with primary
RD were enrolled but 194 were analyzed in the study...
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