Title
Раманова спектроскопија фармаколошки активних супстанци и биокатализатора
Creator
Lazarević, Jasmina J., 1985-, 57142281
Copyright date
2019
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 26.06.2020.
Other responsibilities
mentor
Bugarski, Branko, 1957-, 12474215
član komisije
Knežević-Jugović, Zorica, 1969-, 12781159
član komisije
Bugarski, Diana, 1956-, 1665383
član komisije
Popović, Zoran V., 1952-, 8073575
Academic Expertise
Tehničko-tehnološke nauke
University
Univerzitet u Beogradu
Faculty
Tehnološko-metalurški fakultet
Alternative title
Raman spectroscopy of pharmacologically active compounds and biocatalysts
Publisher
[Јасмина Ј. Лазаревић]
Format
82 стр.
description
Tehnološko-inženjerstvo - Biohemijsko inženjerstvo i biotehnologija /Technological engineering
Research area: Biochemical engineering and biotechnology
Abstract (sr)
U ovoj disertaciji Ramanova spektroskopija korišćena je za sveobuhvatnu analizu primarnih mezenhimskih matičnih ćelija, poreklom iz periodoncijuma, mekog vezivnog tkiva koje spaja koren zuba za alveolarnu kost i ima ulogu u održavanju mineralizacije i ublažavaanja sila koje se javljaju žvakanjem...
Abstract (en)
In this dissertation, the utilization of Raman spectroscopy for comprehensive
analysis of primary mesenchymal stem cells from periodontal ligament, a soft connective
tissue which anchors tooth root for the alveolar bone, was demonstrated.
Since every cell has the unique Raman spectrum, Raman spectroscopy was used
with the purpose to gain the insight in intrinsic biochemical prole on a single cell
level. Mesenchymal stem cells from periodontal ligament were stimulated to dierentiate
towards osteogenic, chondrogenic, and adipogenic lineages. These dierentiated
cells were investigated by means of Raman spectroscopy as well. Microscopic
glass cover-slips were used as substrates due to its cost-eectiveness and common
use in laboratories, which is important for its clinical application. On the other
hand, glass has strong Raman response, but it was demonstrated that the analysis
was possible by taking into account certain spectral region in which its inuence was
eliminated. In all sample groups, the same type of behavior was noticed, manifested
as the increase of relative intensity Raman band of proteins/lipids, and the decrease
of relative intensity of nucleic acids Raman bands. Further, the extensive statistical
analysis in the form of principal component analysis was performed, revealing signi-
cant groupings of cells with the same spectral features, which conrmed previously
obtained results. Despite the heterogeneity of primary mesenchymal stem cells and
their dierentiated lineages, it was proved that micro-Raman spectroscopy could
adequately distinguish cell status, which is important for its clinical and medical
use...
Authors Key words
Ramanova spektroskopija, mezehnimske matične ćelije, analiza pojedinačnih ćelija, polimorfizam, međumolekularne vibracije
Authors Key words
Raman spectroscopy, Mesenchymal stem cells, Single cell analysis, Polymorphism,
Intermolecular vibrations
Classification
543.42:577.27(043.3)
Type
Tekst
Abstract (sr)
U ovoj disertaciji Ramanova spektroskopija korišćena je za sveobuhvatnu analizu primarnih mezenhimskih matičnih ćelija, poreklom iz periodoncijuma, mekog vezivnog tkiva koje spaja koren zuba za alveolarnu kost i ima ulogu u održavanju mineralizacije i ublažavaanja sila koje se javljaju žvakanjem...
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