Title
Ticks fauna and identification and characterization of tick-borne pathogens in red fox populations (Vulpes vulpes) from Serbia
Creator
Juwaid, Salem, 1971-, 25097831
Copyright date
2020
Object Links
Select license
Autorstvo-Nekomercijalno-Bez prerade 3.0 Srbija (CC BY-NC-ND 3.0)
License description
Dozvoljavate samo preuzimanje i distribuciju dela, ako/dok se pravilno naznačava ime autora, bez ikakvih promena dela i bez prava komercijalnog korišćenja dela. Ova licenca je najstroža CC licenca. Osnovni opis Licence: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/deed.sr_LATN. Sadržaj ugovora u celini: http://creativecommons.org/licenses/by-nc-nd/3.0/rs/legalcode.sr-Latn
Language
English
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 30.09.2020.
Other responsibilities
mentor
Tomanović, Snežana, 1972-, 10137447
mentor
Ćirović, Duško, 1968-, 12756327
član komisije
Petrović, Anđeljko, 1981-, 7745127
član komisije
Poluga, Jasmina, 1963-, 56969225
Academic Expertise
Prirodno-matematičke nauke
Academic Title
-
University
Univerzitet u Beogradu
Faculty
Biološki fakultet
Alternative title
Фауна крпеља и идентификација и карактеризација крпељима преносивих патогена у популацијама лисице (Vulpes vulpes) у Србији
Publisher
[S. Juwaid]
Format
76 str.
description
Biology - Entomology / Биологија
- Ентомологија
Abstract (en)
Ticks have a great importance in human and veterinary medicine but general knowledge about tick fauna in different hosts in Serbia is lacking. Since research on ticks that parasitize foxes has not been done systematically, the goal of this study was to research the tick fauna in Red Fox population and identify the most important tick-borne pathogens that can be transmitted to other mammals, including humans. For the purpose of this study 129 red foxes and 113 ticks parasitizing on them were collected. A total of six tick species was identified. The most abundant species was Ixodes ricinus (69%), followed by I. hexagonus (11.5%), I. canisuga (5.3%), I. kaiseri (5.3%), Dermacentor reticulatus (4.4%) and Haemaphysalis concinna (4.4%). Spleen samples from 129 collected red foxes were individually tested for presence of following tick-borne pathogens: Anaplasmataceae, Hepatozoon spp., Babesia spp., Borrelia burgdorferi sensu lato, Rickettsia spp., Coxiella burnetii, Francisella tularensis and Bartonella spp. DNA of pathogenic microorganisms was detected in spleen samples of 94 out of 129 animals (72.9%). DNA of Hepatozoon spp. was detected in 79 analyzed animals (61.2%). H. canis was identified after sequencing and analyzing the data. DNA of Babesia spp. was detected in 38 analyzed animals (29.5%). Presence of two Babesia species was confirmed: B. vulpes in 37 samples (28.7%) and B. canis in one sample (0.8%). Borrelia burgdorferi s. l. complex revealed presence of the pathogen in 7 samples (5.4%) from 5 localities. The obtained sequences from sequencing of 5S-23S rRNA intergenic spacer region confirmed presence of three B. burgdorferi s. l. species: B. burgdorferi s. s., B. lusitaniae and B. garinii. Finally Candidatus Neoerlichia sp. (FU98) was confirmed in 6 Red Fox individuals.
Abstract (sr)
Крпељи имају велики значај у хуманој и ветеринарској медицини, али генерално недостају информације о фауни крпеља код различитих домаћинима у Србији. Будући да истраживање на крпељима који паразитирају на лисицама није рађено систематски, циљ ове студије је био да истражити фауну крпеља у популацији лисице, и да идентификује најважније патогене који преносе крпељи, а који се се могу пренети на друге сисаре, укључујући и људе. За потребе ове студије прикупљено је 129 лисица и 113 крпеља који паразитирају на њима. Идентификовано је укупно шест врста крпеља. Ixodes ricinus (69%), је била најбројнија врста, и прате је I. hexagonus (11.5%), I. canisuga (5.3%), I. kaiseri (5.3%), Dermacentor reticulatus (4.4%) и Haemaphysalis concinna (4.4%). Прикупљени узорци слезине од 129 лисица појединачно су тестирани на присуство следећих патогена који преносе крпељи: Anaplasmataceae, Hepatozoon spp., Babesia spp., Borrelia burgdorferi sensu lato, Rickettsia spp., Coxiella burnetii, Francisella tularensis и Bartonella spp. ДНК патогених микроорганизама је детектована у узорцима слезине код 94 од 129 анализираних животиња (72.9%). ДНК пореклом од Hepatozoon spp. jе детектована у 79 анализираних животиња (61.2%). H. canis је идентификован након секвенцирања и анализирања података. ДНК Babesia spp. је детектована код 38 анализираних животиња (29.5%). Потврђено је присуство две врсте Babesia: B. vulpes код 37 узорака (28.7%), и B. canis у једном узорку (0.8%). Присуство Borrelia burgdorferi s. l. комплекса је откривено код 7 узорака (5.4%) на 5 локалитета. Секвенцирањем добијених секвенци 5S-23S rRNA интергенског региона потврђено је присуство три B. burgdorferi s. l. врсте: B. burgdorferi s. s., B. lusitaniae и B. garinii. Коначно, Candidatus Neoerlichia sp. (FU98) је потврђена код 6 јединки лисица.
Authors Key words
Ticks, tick-borne pathogens, Red Fox, Vulpes vulpes, Hepatozoon spp, Babesia spp, Borrelia spp, Anaplasmataceae
Authors Key words
крпељи, крпељски преносиви патогени, лисица, Vulpes vulpes, Hepatozoon spp, Babesia spp, Borrelia spp, Anaplasmataceae
Classification
596.42:616.9(497.11)(043.3)
Type
Tekst
Abstract (en)
Ticks have a great importance in human and veterinary medicine but general knowledge about tick fauna in different hosts in Serbia is lacking. Since research on ticks that parasitize foxes has not been done systematically, the goal of this study was to research the tick fauna in Red Fox population and identify the most important tick-borne pathogens that can be transmitted to other mammals, including humans. For the purpose of this study 129 red foxes and 113 ticks parasitizing on them were collected. A total of six tick species was identified. The most abundant species was Ixodes ricinus (69%), followed by I. hexagonus (11.5%), I. canisuga (5.3%), I. kaiseri (5.3%), Dermacentor reticulatus (4.4%) and Haemaphysalis concinna (4.4%). Spleen samples from 129 collected red foxes were individually tested for presence of following tick-borne pathogens: Anaplasmataceae, Hepatozoon spp., Babesia spp., Borrelia burgdorferi sensu lato, Rickettsia spp., Coxiella burnetii, Francisella tularensis and Bartonella spp. DNA of pathogenic microorganisms was detected in spleen samples of 94 out of 129 animals (72.9%). DNA of Hepatozoon spp. was detected in 79 analyzed animals (61.2%). H. canis was identified after sequencing and analyzing the data. DNA of Babesia spp. was detected in 38 analyzed animals (29.5%). Presence of two Babesia species was confirmed: B. vulpes in 37 samples (28.7%) and B. canis in one sample (0.8%). Borrelia burgdorferi s. l. complex revealed presence of the pathogen in 7 samples (5.4%) from 5 localities. The obtained sequences from sequencing of 5S-23S rRNA intergenic spacer region confirmed presence of three B. burgdorferi s. l. species: B. burgdorferi s. s., B. lusitaniae and B. garinii. Finally Candidatus Neoerlichia sp. (FU98) was confirmed in 6 Red Fox individuals.
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