Metabolomics approach to quality control of medicinal herbs and herbal products

[S. Ivanović]

114 str.

Hemija - Organska hemija /

Predmet proučavanja ove disertacije bio je ispitivanje biljnih vrsta Origanum vulgare (vranilova trava), O. onites (kritski origano), Allium ursinum (sremuš), Aronia melanocarpa (aronija) i Tribulus terrestris (babin zub) metabolomičkim pristupom. Identifikovana su jedinjenja koja su potencijalni biomarkeri adulteracije i autentičnosti ovih biljnih vrsta. Optimizovane su i razvijene metode instrumentalne analize uzoraka biljnog porekla, čiji su rezultati statistički obrađeni multivarijantnom analizom podataka. GC-MS metabolomičkom analizom detektovan je sorbitol kao biomarker adulteracije O. vulgare i O. onites sa maslinom (Olea europaea), hininska i šikiminska kiselina su pokazatelji adulteracije sa venecijanskim sumakom (Cotinus coggygria), dok različita zastupljenost hininske kiseline i fruktoze ukazuju na adulteraciju sa mirtom (Mirtus communis). NMR metabolomičkim otiskom prsta, izoviteksin, vicenin II, azetidin-2-karboksilna kiselina i trigonelin određeni su za biomarkere adulteracije sremuša sa đurđevkom (Convallaria majalis), dok je izoviteksin određen i kao biomarker adulteracije sa kozlacem (Arum maculatum). Primenom HPTLC metabolomičke analize detektovani su biomarkeri adulteracije ploda aronije. Betain je identifikovan kao marker adulteracije aronije sa vinobojkom (Phytolacca americana), petunidin-3-O-(p-kumaroil)-rutinozid-5-O-glukozid aronije sa pomoćnicom (Solanum nigrum), cijanidin-3-O-sambubiozid-5-O-glukozid, cijanidin 3,5-O-diglukozid i cijanidin-3-O-sambubiozid aronije sa zovom (Sambucus nigra). UPLC-MS/MS analizom autentičnih uzoraka biljke babin zub, dobijeni su biomarkeri autentičnosti koji su zatim detektovani i u komercijalnim uzorcima. Rutin i prototribestin su identifikovani kao biomarkeri prisutni u svim analiziranim uzorcima.

The subject of this dissertation was the examination of the plant species Origanum vulgare (wild marjoram), O. onites (Cretan oregano), Allium ursinum (wild garlic), Aronia melanocarpa (chokeberry), and Tribulus terrestris (puncture vine) using a metabolomics approach. Compounds that are potential biomarkers of adulteration and the authenticity of these plant species have been identified. Methods for instrumental analysis of plant samples were optimized and developed, the results of which were statistically processed by multivariate data analysis. By GC-MS metabolomic analysis, sorbitol was detected as a biomarker of adulteration with olive (Olea europea), quinic and shikimic acids are indicators of adulteration with Venetian sumac (Cotinus coggygria), while different levels of quinic acid and fructose indicate adulteration with myrtle (Mirtus communis). Isovitexin, vicenin II, azetidine-2-carboxylic acid, and trigonelline were determined by NMR metabolomic fingerprinting as biomarkers of adulteration of wild garlic with Lily of the valley (Convallaria majalis), while isovitexin as a biomarker of adulteration with Adam and Eve (Arum maculatum). HPTLC metabolomic analysis detected biomarkers of adulteration in chokeberry. Betaine was identified as a marker of adulteration of pokeweed (Phytolacca decandra), petunidin-3-i-(p-coumaroyl)-rutinoside-5-O-glucoside chokeberry with black night shade (Solanum nigrum), cyanidin-3-O-sambubioside-5-O-glucoside, cyanidin 3,5-O-diglucoside, and cyanidin-3-O-sambubioside with elderberry (Sambicus nigra). Through UPLC-MS/MS analysis of puncture vine samples, biomarkers of authenticity were obtained, which were then detected in commercial samples. Rutin and prototribestin were identified as biomarkers present in all analyzed samples.

metabolomika, adulteracija, hromatografija, multivarijantna analiza, Origanum vulgare, Allium ursinum, Convallaria majalis, Arum maculatum, Aronia melanocarpa, Tribulus terrestris

metabolomcs, adulteration, chromatography, multivariate data analysis, Origanum vulgare, Allium ursinum, Convallaria majalis, Arum maculatum, Aronia melanocarpa, Tribulus terrestris

547.9(043.3)

Tekst