Biochemical characteristics, immunohistochemical analysis, and digital image analysis of pathohistological intestinal specimens from children with inflammatory bowel diseases

[V. Makević]

85 str.

Medicina - Fiziološke nauke / Medicine - Physiological sciences

Zapaljenske bolesti creva grupa su hroničnih zapaljenskih poremećaja gastrointestinalnog trakta koju čine Kronova bolest, ulcerozni kolitis UK i neklasifikovani IBD. U četvrtini slučajeva bolest se javlja pre 20-te godine. Ciljevi ovog istraživanja bili su da se u grupama dece obolele od KB i UK i kontrolnoj grupi odrede: vrednosti standardnih hematoloških i biohemijskih parametara u krvi, koncentracija elemenata u serumu i tkivu creva, morfometrijska, fraktalna i GLCM teksturalna analiza digitalizovanih mikrografa creva i ekspresija parametara oksidativnog stresa 4-HNE u tkivu creva. U studiji preseka učestvovalo je 20 dece obolele od KB, 18 dece obolele od UK i 20 kontrola. Ukupni holesterol je bio niži kod obolelih od UK i KB, a HDL holesterol samo kod obolelih od KB u odnosu na kontrolnu grupu. Kreatin kinaza značajno se razlikovala među ispitivanim grupama. Pokazali smo da je u serumu ispitivane dece gvožđe bilo značajno niže kod dece obolele od KB u odnosu na kontrole, dok je Mn u serumu bio niži kod obolelih od KB u odnosu na obolele od UK. Nadalje, u nekim segmentima creva Mg je bio niži u IBD nego u kontrolnoj grupi. Naši rezultati pokazuju i da su u tankom crevu bakar, mangan i cink bili značajno niži kod dece obolele od IBD u odnosu na kontrole, a Mn i kod dece obolele od KB u odnosu na kontrole. Morfometrijska ispitivanja pokazala su da je po veličini crevnih jedara moguće izdvojiti obolele od UK od drugih ispitivanih grupa, a na osnovu njihove cirkularnosti obolele od KB od kontrole. Osim toga, deca obolela od UK imala su statistički značajno višu fraktalnu dimenziju i nižu lakunarnost hromatina ćelijskih jedara debelog creva u odnosu na obolele od KB i kontrole. GLCM teksturalna analiza jedara završnog dela tankog creva pokazala je razliku između jedara obolelih od UK i jedara drugih ispitivanih grupa. Uz par izuzetaka isto je pokazano i u sigmoidnom kolonu. Rektum i ascendentni kolon bili su značajni segmenti za razlikovanje obolelih od KB od kontrolne grupe. Obim ekspresije 4-HNE u epitelnim ćelijama creva obolelih od KB korelirao je sa GLCM teksturalnim parametrima.

Inflammatory bowel disease (IBD), encompassing Crohn's disease (CD), ulcerative colitis (UC), and unclassified IBD is a chronic inflammatory disorder of the gastrointestinal tract. In a quarter of cases, the disease occurs before the age of 20. This research aimed to determine: the values of standard hematological and biochemical parameters in the blood, the concentration of elements in serum and intestinal tissue, morphometric, fractal, and GLCM textural analysis of digitized micrographs of the intestine, and the expression of oxidative parameters of stress 4 Hydroxynonenal (4-HNE) in intestinal tissue in the groups of children suffering from CD and UC and the control group. In this cross-sectional study, we included 20 children with CD, 18 children with UC, and 20 controls. Total cholesterol was lower in patients with UC and CD, and HDL cholesterol only in those with CD compared to the control group. Creatine kinase was significantly different between the investigated groups. The study shows that serum iron was significantly lower in children suffering from CD compared to controls, while serum Mn was lower in children with CD compared to the ones with UC. Furthermore, in some intestinal segments, Mg was lower in IBD than in the control group. Our results also show copper, manganese, and zinc in the small intestine to be significantly lower in children with IBD compared to controls, and Mn in children with CD compared to controls. Morphometric analysis revealed that according to the size of the intestinal nuclei, it is possible to separate patients with UC from other investigated groups. In addition, based on nuclear circularity, patients with CD can be distinguished from controls. Further, children with UC had a statistically significantly higher fractal dimension and lower lacunarity of colon cell nuclei compared to the children with CD and controls. GLCM textural analysis of the terminal ileum nuclei showed a difference between the nuclei of UC patients and other investigated groups. With a few exceptions, the same is shown in the sigmoid colon. The rectum and ascending colon were significant segments for distinguishing patients with CD from the control group. The extent of 4-HNE expression in intestinal epithelial cells of CD patients correlated with GLCM textural parameters.

Zapaljenske bolesti creva, Kronova bolest, ulcerozni colitis, oligoelementi, morfometrijska analiza, fraktalna analiza, GLCM analiza, 4-HNE

Inflammatory bowel diseases, Crohn's disease, ulcerative colitis, trace elements, morphometric analysis, fractal analysis, GLCM analysis, 4-HNE

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