Title
Graphene and nano-structured oxides composites as components of glucose and polyphenols biosensors
Creator
Đurđić, Slađana Z., 1991-
CONOR:
82416393
Copyright date
2021
Object Links
Language
Serbian
Cobiss-ID
Theses Type
Doktorska disertacija
description
Datum odbrane: 16.07.2021.
Other responsibilities
Academic Expertise
Prirodno-matematičke nauke
Academic Title
-
University
Univerzitet u Beogradu
Faculty
Hemijski fakultet
Alternative title
Композити графена и наноструктурисаних оксида као компоненте биосензора глукозе и полифенола
Publisher
[S. Z. Đurđić ]
Format
147 listova
description
Chemistry - Analytical Chemistry
description
Хемија - Аналитичка хемија
Abstract (en)
Present doctoral dissertation describes the development of novel glucose and polyphenolic index biosensors, based on a combination of graphene nanomaterials and nano-structured metal oxides. An in-depth examination of how mentioned nanomaterials separately influence the electrochemical performances of developed biosensors, as well as their synergic effect, is provided within the scope of this dissertation. In order to obtain desired working electrodes, synthesized nanocomposites were applicated on the surface of screen printed carbon electrodes (SPCEs). Cyclic voltammetry and chronoamperometry were electrochemical methods utilized for testing electrochemical performances of synthetized nanocomposites as well as the final biosensors.
Glucose biosensor was based on the modification of graphene nanoribbons (GNR) with Bi2O3 nanoparticles. The synthesized GNR@Bi2O3 nanocomposite was introduced to the surface of SPCE, in order to obtain the working electrode (SPCE/GNR@Bi2O3). Glucose biosensor was prepared by immobilization of glucose-oxidase (GOx), from Aspergillus niger, on the surface of SPCE/GNR@Bi2O3, and followed by coating of the enzyme with Nafion (Naf) solution. Developed SPCE/GNR@Bi2O3/GOx/Naf biosensor has been successfully applied for the quantification of glucose in a honey sample.
Polyphenolic index biosensor was constructed by modification of graphene nanoplatelets (GNP) with MnO2 nanoparticles. In order to produce the working electrode (SPCE/GNP@MnO2), SPCE has been modified with GNP@MnO2 nanocomposite. The final polyphenolic index biosensor has been produced by immobilization of the laccase, from Trametes Versicolor (TvL), onto the SPCE/GNP@MnO2 surface, and followed by the application of Naf. Using the developed SPCE/GNP@MnO2/TvL/Naf biosensor, the polyphenolic index in wine was successfully determined.
Abstract (sr)
Представљена докторска дисертација описује развој нових биосензора за одређивање глукозе и полифенолног индекса који се заснивају на комбинацији графенских наноматеријала и наноструктурисаних металних оксида. Утицај поменутих наноматеријала на електрохемијске перформансе развијених биосензора, као и њихов синергијски ефекат, је детаљно проучен у оквиру ове дисертације. Синтетисани нанокомпозити су нанети на површину штампаних угљеничних електрода (SPCE) у циљу добијања радних електрода. Циклична волтаметрија и хроноамперометрија су електрохемисјке методе које су коришћене за испитивање електрохемијских перформанси синтетисаних нанокомпозита, као и финалних биосензора.
Биосензор за одређивање глукозе се заснивао на модификацији графенских нанотрачица (GNR) са Bi2O3 наночестицама. Синтетисани GNR@Bi2O3 нанокомпозит је нанет на површину SPCE у циљу добијања радне електроде (SPCE/GNR@Bi2O3). Биосензор за одређивање глукозе је припремљен имобилизацијом глукоза-оксидазе (GOx) из Aspergillus niger на површину SPCE/GNR@Bi2O3, што је праћено превлачењем ензима са раствором нафиона (Naf). Развијени SPCE/GNR@Bi2O3/GOx/Naf биосензор је успешно примењен за квантификацију глукозе у узорку меда.
Биосензор за одређивање полифенолног индекса се базирао на модификацији графенских наноплочица (GNP) са MnO2 наночестицама. SPCE је модификована са GNP@MnO2 нанокомпозитом у циљу конструкције радне електроде (SPCE/GNP@MnO2). Биосензор за одређивање полифенолног индекса је конструисан након имобилизације лаказе из Trametes Versicolor (TvL) на површину SPCE/GNP@MnO2, након чега је уследило наношење Naf раствора. Полифенолни индекс у узорцима вина је одређен користећи развијени SPCE/GNP@MnO2/TvL/Naf биосензор.
Authors Key words
biosensors, electrochemistry, glucose, glucose-oxidase, graphene nanoplatelets, graphene nanoribbons, laccase, metal oxide nanoparticles, polyphenolic index
Authors Key words
биосензори, електрохемија, глукоза, глукоза-оксидаза, графенске наноплочице, графенске нанотрачице, лаказа, наночестице металних оксида, полифенолни индекс
Classification
543.272.7:544.6.076(043.3)
Type
Tekst
Abstract (en)
Present doctoral dissertation describes the development of novel glucose and polyphenolic index biosensors, based on a combination of graphene nanomaterials and nano-structured metal oxides. An in-depth examination of how mentioned nanomaterials separately influence the electrochemical performances of developed biosensors, as well as their synergic effect, is provided within the scope of this dissertation. In order to obtain desired working electrodes, synthesized nanocomposites were applicated on the surface of screen printed carbon electrodes (SPCEs). Cyclic voltammetry and chronoamperometry were electrochemical methods utilized for testing electrochemical performances of synthetized nanocomposites as well as the final biosensors.
Glucose biosensor was based on the modification of graphene nanoribbons (GNR) with Bi2O3 nanoparticles. The synthesized GNR@Bi2O3 nanocomposite was introduced to the surface of SPCE, in order to obtain the working electrode (SPCE/GNR@Bi2O3). Glucose biosensor was prepared by immobilization of glucose-oxidase (GOx), from Aspergillus niger, on the surface of SPCE/GNR@Bi2O3, and followed by coating of the enzyme with Nafion (Naf) solution. Developed SPCE/GNR@Bi2O3/GOx/Naf biosensor has been successfully applied for the quantification of glucose in a honey sample.
Polyphenolic index biosensor was constructed by modification of graphene nanoplatelets (GNP) with MnO2 nanoparticles. In order to produce the working electrode (SPCE/GNP@MnO2), SPCE has been modified with GNP@MnO2 nanocomposite. The final polyphenolic index biosensor has been produced by immobilization of the laccase, from Trametes Versicolor (TvL), onto the SPCE/GNP@MnO2 surface, and followed by the application of Naf. Using the developed SPCE/GNP@MnO2/TvL/Naf biosensor, the polyphenolic index in wine was successfully determined.
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