Analysis of the concentration of the selected hematological parameters, C-reactive protein and Anti Mullerian hormone in bitches with pyometra

[D. T. Janković]

106 listova

Veterinarska medicina - Ginekologija sa andrologijom / Veterinary medicine - Ginecology and andrology

Analiza koncentracije izabranih hematoloških parametara, C-reaktivnog proteina i Anti-Milerovog hormona (AMH) kod kuja sa piometrom i kontrolne grupe, ispitivana je kod jedinki različite rase, starosne kategorije, različitog habitualnog statusa, načina ishrane i fertilnog perioda. Cilj rada bio je da se ispitaju klinički status, hematološki, biohemijski parametri, koncentracije proteina akutne faze (CRP, albumi), koncentracije progesterona (PG) i antimilerovog hormona (AMH) kod kuja sa piometrom u poređenju sa zdravim kujama. Takođe, cilj rada je bio da se ispita mogućnost primene nekog od ispitivanih parametara kao mogućeg biomarkera u dijagnostici piometre i praćenju postoperativnog toka. U ogled je bilo uključeno 40 kuja različitih rasa i približne starosti (3-6 godina), koje su podeljene u dve grupe: kontrolnu (C, n = 20) i oglednu grupu, kuje kod kojih je dijagnostikovana piometra (P, n = 20). Uzorci krvi za analizu su uzimani preoperativno (0. dan), 12h, 24h, 48h i 72h nakon hirurške intervencije, osim AMH i PG čije su koncentracije određivane samo 0. dana. Nakon kliničkog pregleda utvrđene su značajno više vrednosti broja udisaja u minuti kod kuja sa piometrom u poređenju sa zdravim kujama u svim ispitivanim vremenima, ubrzan puls 0h i 12h, kao i povišena temperatura 0h, 12h, 48h i 72h (p<0.01). Značajno više koncentracije CRP (p<0,01) i snižene vrednosti albumina utvrđene su kod kuja sa piometrom u poređenju sa zdravim kujama u svim ispitivanim vremenima. Analizom koncentracije AMH neposredno pred operativni zahvat utvrđene su značajno više koncentracije ovog hormona kod kuja sa piometrom u poređenju sa zdravim kujama (p<0.01). Inflamatorna rekcija zapaljenja endometrijuma dovodi do značajnog povećanja koncentracije AMH, dok se povišene koncentracije CRP i snižene vrednosti albumina u toku ispitivanog vremena ukazuju na dijagnostičku specifičnost i mogućnost upotrebe kao biomarkera prozapaljenske aktivnosti, odnosno odgovora akutne faze. Naši rezultati pokazuju da klinički i laboratorijski nalazi, kao i analiza inflamatornih faktora mogu biti korisni za procenu zdravstvenog stanja kuja sa piometrom kao i za procenu i prognozu mogućih komplikacija zdravstvenih stanja nakon hiruškog zahvata i/ili sterilisanja kuja sa piometrom.

Analysis of the concentration of hematological parameters, C-reactive protein and Anti-Mullerian hormone (AMH) in bitches with pyometra and control group, was investigated in individuals of different breeds, age categories, different habit status, diet and fertile period.The aim of the work was to investigate clinical status, hematological, biochemical parameters, concentrations of acute phase proteins (CRP, albumins), concentrations of progesterone (PG) and Anti-Mullerian hormone (AMH) in bitches with pyometra compares to healthy bitches.. The goal of the work was also to investigate the possibility of applying some of the investigated parameters as possible biomarkers in the diagnosis of pyometra and monitoring the postoperative course. 40 bitches of different breeds and approximate age (3-6 years) were included in the experiment, which were divided into two groups: control (C, n = 20). Blood samples for analysis were taken preoperatively (day 0), 12 hours, 24 hours, 48 hours, and 72 hours after surgery, except for AMH and PG, whose concentrations were determined only on day 0. After the clinical examination, significantly higher values of the number of breaths per minute in bitches with pyometra compared to healthy bitches at all examined times, increased pulse at 0h and 12 h, and elevated temperature at 0h, 12h, 48h and 72h (p<0.01). Significantly higher concentrations of CRP (p<0.01) and decreased albumin values were found in bitches with pyometra compared to healthy bitches at all time points examined. Analysis of the AMH concentration immediately before surgery showed significantly higher concentrations of this hormone in bitches with pyometra compared to healthy bitches (p<0.01). Inflammatory reaction of endometrial inflammation leads to a significant increase in AMH concentration, while elevated CRP concentrations and decreased albumin values during the investigated period indicate diagnostic specificity and the possibility of use as a biomarker of pro-inflammatory activity, i.e. acute phase response. Our results show that clinical and laboratory findings, as well as analysis of inflammatory factors can be useful for assessing the health status of bitches with pyometra, as well as for the assessment and prognosis of possible complications of health status after surgery and/or spaying of bitches with pyometra.

kuja, piometra, hematološki parametri, C-reaktivni protein, AMH

bitch, pyometra, hematological parameters, C-reactive protein, AMH

636.7.09:616-07(043.3)

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